Your answer should be typed (Calibri 11-font or equivalent) and must fit on no more than 2 letter-sized pages with 1″ margins.
Your lab is equipped with HPL-UV, UPL-MS, GC-MS (single quadrupole), GC-ECD, and GC-FID. Your GC-MS can operate in El and Cl (methane) mode. You may use scan, SIM, or both. The C’s have split/splitless injectors. You also have options for sample prep and extraction including SPE, Soxhlet extractors, separatory funnels, rotovap, and appropriate volumetric glassware (flasks, pipettes, syringes). You have both reverse phase and normal phase semi-preparative and analytical HPLC columns and reverse phase analytical UPC columns. Your GC column inventory is limited to one manufacturer { ) but includes: DB-5 columns (low-polarity, (5%-Phenyl)-methy|polysiloxane); DB-1701 columns (mid- to low-polarity (14%-Cyanopropyl-phenyl)-methylpolysiloxane); and DB-WAX columns (high-polarity polyethylene glycol (PEG)).
A container of 10 L of river water was delivered to your lab. It is likely that there are trace quantities of chlorinated pesticides, PCB, PAH, low molecular weight hydrocarbons (including aromatics), phenols, and glycols in the contaminated water. You must qualitatively and quantitively identify chlorinated pesticides present in the part-per-trillion range. Describe your procedures using any or all the instrumentation in your lab. Justify your approach.
Properly cite any references used.
HINT: “No need to reinvent the wheel.” Start with a literature search to learn how others have extracted and analyzed chlorinated pesticides in water.
